It can be tricky to have a perfect silence in an experimental space, even if soundproofed. But it is required especially when low sound-level communications are studied such as with arthropods. One of the issues I have had is the sound generated by some electric or electronic devices which needs to be present in the experimental room; despite their very low levels, I was able to hear high-pitched sounds when putting my ears very close to the device or the bulb.
What kind of light do you use to be sure that your light system does not become a source of sound, whatever low level it is?